Process for isolating Cu,Zn-superoxide dismutase from aqueous so

Details Process for isolating Cu,Zn-superoxide dismutase from aqueous so Process for isolating Cu,Zn-superoxide dismutase from aqueous so

1981-10-27

1983-06-14

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435192, 435814, 435815, C12N 902, C12N 908

Patent

active

043884067

ABSTRACT:
Cu,Zn-superoxide dismutase (SOD) is isolated from aqueous solutions containing said enzyme together with accompanying proteins by chromatography of the solution at a pH of 4.7 to 5.5 on a cation exchange resin of the same polarity as SOD in the pH range used. As cation exchange resin may particularly be used carboxymethyl celluloses, cross-liked dextrans substituted with carboxymethyl groups or sulfopropyl groups or cross-linked agaroses substituted with carboxymethyl groups.
The process lends itself to use on an industrial scale and provides a high yield of pure SOD.

REFERENCES:
patent: 3758682 (1973-09-01), Huber et al.
Goscin et al., Biochim. Biophys. Acta vol. 289, pp. 276-283 (1972).

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