Process for the production of carrier particles from yeast cells

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435174, 435175, 435255, 435810, 424 12, 23230B, G01N 3354

Patent

active

043201946

ABSTRACT:
Yeast cells are converted into carrier particles useful as carriers for proteins and other materials useful in agglutination tests by cross-linking the proteins of the cell cytoplasm followed by stabilization of the carbohydrates of the cell wall by reaction with an epoxide. The stabilized cells obtained may be dyed and may be esterified or etherified to block the hydroxyl groups in their surfaces. They may be coupled to the protein or other material by activation, e.g. with cyanuric chloride, followed by reaction with the protein or other material to produce diagnostic agents useful in test kits.

REFERENCES:
patent: 3553310 (1971-01-01), Cszimas et al.
patent: 3639558 (1972-02-01), Cszimas et al.
patent: 3652761 (1972-03-01), Weetall
patent: 3987159 (1976-10-01), Spona et al.
patent: 4107287 (1978-08-01), Morton et al.

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