Method and kit for detecting betalactam-containing compounds

Details Method and kit for detecting betalactam-containing compounds Method and kit for detecting betalactam-containing compounds

1999-06-23

2000-11-07

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 23, 435 71, 435 4, C12Q 137, C12Q 100, G01N 3353

Patent

active

061435139

ABSTRACT:
Methods and kits are disclosed for detecting betalactam-containing compounds in a sample, and typically liquid products intended for human consumption such as milk. Betalactam-containing compounds include the class of betalactam antibiotics which may be present as undesired contaminates of milk. The methods involves contacting the sample with a D,D-carboxypeptidase and a substrate having a carboxyterminal D-alanine-D-alanine to form a reaction mixture, wherein the D,D-carboxypeptidase is capable of cleaving the terminal D-alanine from the substrate to yield a cleaved substrate. In an optional step, the reaction mixture is further contacted with a ligand specific to the cleaved substrate or substrate. The cleaved substrate or substrate of the reaction mixture, or the ligand specific to the cleaved substrate or substrate, is then captured by a surface-bound binding partner, with the amount of captured material being indicative to the amount of betalactam-containing compound in the sample. Kits for performing the methods of this invention are also disclosed.

REFERENCES:
patent: 4546076 (1985-10-01), Degelaen et al.
patent: 4686182 (1987-08-01), Drake
Frere et al., "Enzymatic Method for Rapid and Sensitive Determination of .beta.-Lactam Antibiotics," Antimicrobial Agents And Chemotherapy 18(4):506-510, 1980.

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