Process for purifying DNA and RNA using cationic detergents

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 28, 536 29, 435262, 935 19, 935 20, C07H 2100, C07H 2102, C07H 2104

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active

050101838

ABSTRACT:
A novel method is provided for purifying DNA and RNA from a variety of sources, including cells, cell lysates, viruses, tissues, blood and other body fluids employing a cationic detergent to complex with the nucleic acids.

REFERENCES:
patent: 3152116 (1964-10-01), Broida
patent: 3157637 (1964-11-01), Khym
patent: 3845033 (1974-10-01), Harnden
patent: 4018916 (1977-04-01), Hodge
patent: 4833239 (1989-05-01), DeBonville et al.
patent: 4843155 (1989-01-01), Chomczynski
J. F. Robyt et al., Biochemical Techniques, Theory and Practice, Brooks/Cole Publishing Co., Monterey, CA, 1987, pp. 254-255.
Robinson, "The Organic Constituents of Higher Plants", 5th ed, Cordus Press, Ninth Amherst, MA, 1983, see pp. 270-272.
Davis et al., "Rapid DNA Isolation for Enzymatic and Hybridization Analysis", Ch. 49 in vol. 65.
"Methods in Enzymology" Grossman et al., eds., Academic Press, New York, New York, 1980, see pp. 404-411, esp. p. 405.
Perbal, "A Practical Guide to Molecular Cloning", John Wiley & Sons, New York, New York, 1984, pp. 152-159, esp. pp. 158-159.
Weil et al., Biochem. Biophys. Acta., 55:836-840 (1962).
Weil et al., Nature, 4798(192):169 (1961).
Jones, Biochem. et Biophys. Acta., 10;607-612 (1953).
Jones, Nature, 199:280 (1963).
Manfioletti et al., Nucleic Acids Research, 7(16):2873-2884 (1988).
Ralph et al., Biochem. Biophys. Acta., 87:9-16 (1964).
Aubel-Sadron et al., Biochem. Biophys. Acta., 53:11-18 (1961).

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