Isolated nucleic acid molecules encoding antigen binding sites o

Details Isolated nucleic acid molecules encoding antigen binding sites o Isolated nucleic acid molecules encoding antigen binding sites o

1995-06-07

1998-09-22

Feisee, Lila

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 7021, 4351721, 4351723, 536 231, 536 234, 5303871, 5303873, 5302877, 5303888, 53038885, C12P 2104, C07K 1600, C07H 2104

Patent

active

058112677

ABSTRACT:
Novel compositions are provided that are derived from antigen-binding sites of immunoglobulins having affinity for cancer antigens. The compositions exhibit immunological binding properties of antibody molecules capable of binding specifically to a human tumor cell displaying a MDR phenotype. A number of synthetic molecules are provided that include CDR and FR regions derived from same or different immunoglobulin moieties. Also provided are single chain polypeptides wherein V.sub.H and V.sub.L domains are attached by a single polypeptide linker. The sFv molecules can include ancillary polypeptide moieties which can be bioactive, or which provide a site of attachment for other useful moieties. The compositions are useful in specific binding assays, affinity purification schemes, drug or toxin targeting, imaging, and genetic or immunological therapeutics for various cancers. The invention thus provides novel polypeptides, the DNAs encoding those polypeptides, expression cassettes comprising those DNAs, and methods of inducing the production of the polypeptides.

REFERENCES:
patent: 4753894 (1988-06-01), Frankel et al.
patent: 4912039 (1990-03-01), Riordan
patent: 4938948 (1990-07-01), Ring et al.
patent: 4946778 (1990-08-01), Ladner et al.
patent: 4956453 (1990-09-01), Bjorn et al.
patent: 5091513 (1992-02-01), Huston et al.
patent: 5132405 (1992-07-01), Huston et al.
patent: 5206352 (1993-04-01), Roninson et al.
Hanada et al, Cancer Res. 50: 3167-3171, 1990.
Reeck et al, Cell, 50:667, 1987.
Lewin, Science, 1988, 237: 1570.
Alberts, MolBio of the Cell, Garland Publishing, NY, 1983, pp. 965-966.
Huston et al., "Protein Engineering of Antibody Binding Sites: Recovery of Specific Activity in an Anti-digoxin Single-chain Fv Analogue Produced in Escherichia coli," Proc. Natl. Acad. Sci. (USA) (1988) 85:5879-5883.
Riechmann et al., "Reshaping Human Antibodies for Therapy," Nature (1988) 332:323-327.
Verhoeyen et al., "Reshaping Human Antibodies:Grafting an Antilysozyme Activity," Science (1988) 239:1534-1536.

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