Preparation of natural or modified insect toxins

Details Preparation of natural or modified insect toxins Preparation of natural or modified insect toxins

1985-03-28

1991-04-23

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

536 27, 4353201, 43525233, 4351723, 935 6, 935 44, C12N 121, C12N 1500, C12N 1532

Patent

active

050100017

ABSTRACT:
Enhanced production of B. thuringiensis delta-endotoxin is achieved by employing the naturally occurring gene where an abbreviated 3'- and 5'-non-coding flanking region is employed. Particularly, the 3'-flanking does not extend past the downstream HincII cleavage site and the 5'-flanking region does not extend past the upstream HincII cleavage site. By having abbreviated flanking regions, enhanced production of the delta-endotoxin in E. coli is obtained.

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Wong, S. H. et al., Proc. Natl. Acad. Sci., vol. 81, pp. 1184-1188, Feb., 1984.
Whiteley, H. R. et al., in Molecular Cloning and Gene Regulation in Bacillus, (Ganesan Chang, Hoch, Eds.) pp. 131-144, 1982.
Moran, C. P. et al. Mol. Gen. Genet, vol. 186, pp. 339-346, 1982.
Held, G. A. et al., Proc. Natl. Acad. Sci., vol. 79, pp. 6065-6069, 1982.

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