Process for separating ribonucleic acids from a solution contain

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 28, 536 29, C07H 1512, C07H 1700

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046237232

ABSTRACT:
Crude nucleic acid solutions can be selectively separated using a membrane separating process, it being possible for the high molecular weight DNA to be isolated from the retentate and the low molecular weight RNA to be isolated from the permeate. Ultrafiltration on hollow fiber membranes is preferred.

REFERENCES:
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patent: 4482482 (1984-11-01), Haff et al.
Ahonen et al., Separation of tRNA from DNA by Gel Filtration, Chem. Abstracts 65: 20411g (1966).
Ikeguchi, Membrane Separation, Chem. Abstracts 88: 107401a (1977).
Freifelder, Physical Biochemistry, pp. 143 and 220-221 (1976).
Hombach, Molecular Size Distributions of Coal Derivatives, Chem. Abstracts 96: 125956e (1982).
Toyomoto, Hollow Fiber-Type Ultrafiltration Systems, Chem. Abstracts 88: 172457n (1977).
Breslau et al., Advances in Hollow Fiber Ultrafiltration Technology, Chem. Abstracts 94: 141725t (1980).
Berns et al., Isolation of High Molecular Weight DNA from Haemophilus Influenzae, Chem. Abstracts 63: 2033c (1965).
Staude, Current Status of Separation Technology with Membranes, Chem. Abstracts 91: 22958z (1979).

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