Simultaneous quantitative immunoassay for different antigens or

Details Simultaneous quantitative immunoassay for different antigens or Simultaneous quantitative immunoassay for different antigens or

1983-10-31

1986-11-18

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 7, 436513, 436519, 436520, 436522, 436543, 436547, 436523, 436819, 436829, C12Q 168, G01N 3354

Patent

active

046236180

ABSTRACT:
An immunoassay by which a plurality of kinds of antigens or antibodies in one test sample may be simultaneously quantified. Each of a plurality of kinds of microcapsules is first provided for each kind of a plurality of antigens or antibodies to be quantified such that the microcapsules bind an antibody or antigen specific to one kind of the plurality of antigens or antibodies to be quantified. The microcapsules are formed of a membrane capable of being lysed by the complement activity, and each kind of microcapsules contains therein a substance that is quantifiable and does not interact with another quantifiable substance contained in other kinds of microcapsules. The plurality of kinds of microcapsules are mixed with a test sample and complement, and the quantifiable substances are released from the microcapsules upon lysis of the microcapsules by the complement activity. The quantifiable substances are then quantified.

REFERENCES:
patent: 3850578 (1974-11-01), McConnell
patent: 4235792 (1980-11-01), Hsia et al.
patent: 4292403 (1981-09-01), Duermeyer
patent: 4338094 (1982-07-01), Elahi
patent: 4342826 (1982-08-01), Cole
Haxby-Proc. N.A.S., vol. 61 (1968), pp. 300-307.
D'Orazlo et al.-Anal. Chem., vol. 49, No. 13 (Nov. 1977), pp. 2083-2086.
Cole-International Patent Document-WO81/02344, (Aug. 20, 1981), pp. 1-43.

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