Sheets, kits and methods for detecting antigens or antibodies

Details Sheets, kits and methods for detecting antigens or antibodies Sheets, kits and methods for detecting antigens or antibodies

1996-11-12

1998-10-27

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 56, 422 57, 435 794, 435 795, 4352872, 4352877, 4352879, 435969, 435970, 435975, 436524, 436808, G01N 33551

Patent

active

058276696

ABSTRACT:
A detection sheet capable of detecting an antigen or antibody in a biological fluid with a high sensitivity in a simple operation, as well as a detection kit and detection method using the detection sheet. The detection sheet comprises (a) a fibrous aggregate with carried particles of a calcium phosphate compound having an average particle diameter of about 0.01 to 200 microns and a Ca/P ratio of about 1.0 to 2.0, and (b) an avidin, streptavidin or avidin derivative from which carbohydrates have been extracted immobilized on said calcium phosphate compound. After a biotinylated antigen or antibody is bonded to the immobilized avidin, streptavidin, or avidin derivative of the detection sheet, the detection sheet is contacted with a test solution to thereby bond an antigen or antibody of the test solution to the detection sheet. The detection sheet is then contacted with a solution of labeling compound capable of being specifically bonded to the antigen or antibody of said test solution to detect the thus labeled antigen-antibody complex.

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WPI Abstract Accession No. 89-088903/12, published 1995.

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