Homocysteine assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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435 18, 435 15, 435 21, 435 71, 435 16, 435 23, 435 24, 435 28, 435975, C12Q 100, C12Q 134, C12Q 148, C12Q 142

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058276459

ABSTRACT:
The invention relates to a method for assaying homocysteine in a sample such as blood, plasma, or urine, which comprises the steps of contacting the sample with a homocysteine converting enzyme and at least one substrate for the enzyme other than homocysteine, and without chromatographic separation, assessing a non-labelled analyte selected from a homocysteine co-substrate and the homocysteine conversion products of the enzymic conversion of homocysteine by said enzyme.

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