Process for sampling and analysis by thin layer chromatography

Chemistry: analytical and immunological testing – Lipids – triglycerides – cholesterol – or lipoproteins

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73 611C, 128749, 2101983, 210658, 422 70, 436162, 436178, G01N 3108, G01N 3392

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active

044382057

ABSTRACT:
Sampling and analyzing at least one mixture of substances by slab chromatography or thin layer chromatography involves sampling the mixture on a non-adsorbent solid support using a sampling member to achieve precisely repeatable conditions, depositing an appropriate amount of the mixture to be analyzed on an edge zone of a chromatography plate, bringing the said edge of the chromatography plate into contact with a solvent or a mixture of solvents to cause the migration of the mixture on the chromatography plate and the separation of the constituents of the said mixture, and developing the said plate for the qualitative and/or quantitative analysis of the constituents of the mixture, by pressing that face of the support which bears the mixture against the chromatography plate until the mixture to be analyzed has essentially migrated entirely out of the abovementioned support.
The action of pressing the support against the chromatography plate is carried out with the aid of a specially designed holding means fastened to the chromatography plate.

REFERENCES:
patent: 3714035 (1973-01-01), Jones
patent: 3785930 (1974-01-01), Ellis
patent: 3915856 (1975-10-01), Meyer
patent: 3963421 (1976-06-01), Jones
patent: 4158626 (1979-06-01), Halpaap et al.
patent: 4377641 (1983-03-01), Dee et al.

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