Polyvalent display libraries

Details Polyvalent display libraries Polyvalent display libraries

1997-04-04

2000-05-02

Huff, Sheela

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435 78, 435 914, 435471, 435489, 435 691, 435 696, 4353201, 536 231, 536 234, 536 2353, C12Q 168, G01N 3353, C12N 1564, C12P 2104

Patent

active

060570989

ABSTRACT:
The invention is directed to inter alia two related but self-sufficient improvements in conventional display methods. The first improvement provides methods of enriching conventional display libraries for members displaying more than one copy of a polypeptide prior to affinity screening of such libraries with a target of interest. These methods can achieve diverse populations in which the vast majority of members retaining full-length coding sequences encode polypeptides having specific affinity for the target. In a second aspect, the invention provides methods of subcloning nucleic acids encoding displayed polypeptides of enriched libraries from a display vector to an expression vector without the need for clonal isolation of individual members. These methods result in polyclonal libraries of antibodies and other polypeptides for use, e.g., as diagnostic or therapeutic reagents.

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