Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1988-12-29
1990-08-28
Nutter, Nathan M.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530384, 530412, 530413, 210656, 210927, 514822, 424530, C07K 320, A61K 3514, A61K 3516
Patent
active
049526758
ABSTRACT:
Disclosed are methods for purifying proteins including AHF by column chromatography in the presence of additives including sugars and polyhydric alcohols which serve to increase the electrostatic forces on the surface of said proteins while decreasing the hydrophobicity of said proteins resulting in preparations of such proteins of high purity and/or resolution and/or recovery.
REFERENCES:
patent: 4348315 (1982-09-01), Blomback et al.
patent: 4387092 (1983-06-01), Liautaud et al.
patent: 4404131 (1983-09-01), Schwarz et al.
patent: 4404132 (1983-09-01), Mitra
patent: 4439357 (1984-03-01), Bonhard et al.
patent: 4508709 (1985-04-01), Amphlett et al.
patent: 4743680 (1988-05-01), Mathews et al.
patent: 4803073 (1989-02-01), Doleschel et al.
Lundblad et al., "The Effect of Dextrose on Chromatography of Antihemophilic Factor (Factor VIII)" Thrombosis Research, 1:197, 1972, pp. 197-200.
J. of Biochemistry, vol. 90, No. 1, Jul. 1981 (Gekko et al.) pp. 39-50.
Archives of Biochem. & Biophysics, vol. 224, 1983 (Arakawa et al.) pp. 169-177.
Austen et al., "Factor VIII Fractionation on Aminohexyl Sepharon with Possible Reduction in Hepatitis B Antigen", Thromb. Haemstas 48(1):46 (1982).
Faure et al., "Improved Buffer for the Chromatographic Separation of Factor VIII Coagulant", J. Chromatog:257:387 (1983).
Johnson Alan J.
Mathews Rita W.
New York University
Nutter Nathan M.
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