Method of preparing alpha-1-proteinase inhibitor

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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530416, 530421, 530424, 530831, 424101, 210660, 210670, 210905, 210927, C07G 700

Patent

active

046970037

ABSTRACT:
There is disclosed a method for separating alpha-1-proteinase inhibitor (also known as alpha-1 antitrypsin) from an aqueous solution of plasma proteins, especially from Cohn Effluent II & III and Cohn Effluent I. The method includes the steps of first treating the aqueous solution to lower the concentration of salts therein and, optionally, its alcohol content, contacting the resulting solution with an anion exchange resin having selective affinity for alpha-1-proteinase inhibitor to selectively bind the alpha-1-proteinase inhibitor and allow unwanted plasma proteins to elute through the resin, displacing the alpha-1-proteinase inhibitor from the resin and recovering the same.

REFERENCES:
patent: 3415804 (1968-12-01), Polson
patent: 3869436 (1975-03-01), Falksveden
patent: 4100149 (1978-07-01), Meiller et al.
patent: 4379087 (1983-04-01), Coan et al.

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