Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1986-07-14
1989-10-24
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 24, 435 26, 435 41, 435174, 435176, 435178, 435180, 436518, 436524, 436531, 436548, G01N 33573, G01N 33543, C12P 100, C12N 1100
Patent
active
048761915
ABSTRACT:
The invention relates to compositions which are capable of selectively binding a biologically active protein, retaining its activity. Such compositions have essentially three parts, linked together: a water-insoluble carrier, an antibody bound by the carrier and being against the Fc region of an immunoglobulin and an antibody bound thereto which is specific towards the protein which is to be bound to this composition. Proteins of choice are enzymes and isoenzymes such as carboxypeptidas A or isoenzyme 5 of porcine lactate dehydrogenase. Preferably the antibody bound to the carrier is a polyclonal anti-mouse antibody and the antibody bound thereto is a monoclonal mouse antibody. Based on such compositions, there is provided an assay for the quantitative determination of such proteins.
REFERENCES:
patent: 4002532 (1977-01-01), Weltman et al.
patent: 4048298 (1977-09-01), Niswender
patent: 4433059 (1984-02-01), Chang et al.
patent: 4693985 (1987-09-01), Degen et al.
Srivastara, et al., Journal of Biochemical and Biophysical Methods, vol. 2, 1980, pp. 1-9.
"Immunodiffusion and Immunoelectrophoresis", O. Ouchterlony et al., in Handbook of Experimental Immunology, D. M. Weir, Ed., vol. 1, pp. 19.1-19.5, Blackwell, Canada, 1978.
"The Kinetics of Some Carboxypeptidase A and Acetylcarboxypeptidase A Catalyzed Hydrolses," J. R. Whitaker et al., Biochemistry, vol. 5, No. 1, 1968, pp. 386-392.
"A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding," M. M. Bradford, Analytical Biochemistry, 72, 248-254, 1976.
"Immunoassay Using Antigen-Enzyme Conjugates," B. K. Van Weeman et al., FEBS Letters, vol. 15, No. 3, pp. 232-236, Jun. 1971.
"Continous Cultures of Fused Cells Secreting Antibody of Predefined Specificity," G. Kohler et al., Nature, vol. 256, pp. 495-497, Aug. 7, 1975.
"A Rapid Method of Preparing Pure Serum Gamma-Globulin," D. R. Stanworth, Nature, vol. 188, pp. 156-157, Oct. 8, 1960.
"Interaction of Carboxypeptidase A with Monoclonal Antibodies," Beka Solomon et al., Molecular Immunlogy, vol. 21, No. 1, pp. 1-11, 1984.
"High Frequencies of Antigen-Specific Hybridomas: Dependence on Immunization Parameters and Prediction by Spleen Cell Analysis," C. Stahli et al., Journal of Immunological Methods, 32 (1980) 297-304.
"Preparation of Adsorbents for Biospecific Affinity Chromatography . . . " L. Sundberg et al., Journal of Chromatography, 90 (1974) 87-98.
"Nephelometric Activity as a Criterion of Adequate Antisera for use in Immunofluorescence," J. Gauldie, et al., Int. Archs Allergy Appl. Immun. 60: 186-194 (1979).
"Isolation of Pure IgG.sub.1, IgG.sub.2a and IgG.sub.2b Immunoglobulins from Mouse Serum Using Protein A-Sepharose," P. L. Ey et al., Immunochemistry, vol. 15, pp. 429-436, 1978.
"Lactate Dehydrogenase," in the Enzymes, 3rd Ed., P. O. Boyer, Ed., vol. XI, pp. 191-292, 1975.
Hollander Zeev
Katchalski-Katzir Ephraim
Salomon Beka
Naff David M.
Ramot University Authority for Applied Research and Industrial
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