Process for the in vitro immunization of human splenocytes again

Details Process for the in vitro immunization of human splenocytes again Process for the in vitro immunization of human splenocytes again

1985-05-22

1992-04-21

Nucker, Christine

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524031, 43524021, 424 88, C12N 502, A61K 3900

Patent

active

051067463

ABSTRACT:
A process for the in vitro immunization of an immuno-competent splenocyte against an immunogen comprising (a) obtaining a first human splenocyte population, (b) fractionating the first population so that the first fraction is enriched with T-cells and a second fraction is enriched with B-cells, (c) mixing together the cells from the first and second fractions to form a second population having a T-helper cell to B-cell ratio of at least 0.4, and the percentage of T-suppressor cells is essentially unchanged from the first population, and (d) culturing the second population in a medium containing human serum, an immunogen and a lymphokine or lymphokines which induce proliferation and differentiation of T and B cells.

REFERENCES:
patent: 4668629 (1987-05-01), Kaplan
Dosch, H. and Gelfand, E. W., Journal of Immunology, vol. 118, No. 1, pp. 302-308, 1977.
Mishell & Dutton, "Immunization of Dissociated Spleen Cell Cultures from Normal Mice", J. Exp. Med. 126:423 (1967).
Borrebaeck et al., Chem. Abs. 44110q, vol. 102, No. 5, p. 425, Feb. 4, 1985.
Borrebaeck, Acta Chemica Scandinavica, pp. 647-648, vol. B37, No. 7 (1983).
Ho & Durda, J. Cell. Bio., Supp. No. 9, Part A, p. 41, No. 0090 (1985).

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