Process for the preparation of immunologically active enzyme tag

Details Process for the preparation of immunologically active enzyme tag Process for the preparation of immunologically active enzyme tag

1982-05-19

1984-12-04

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

435 7, 435177, 435178, 436543, 436544, 436547, G01N 3354, C12N 996

Patent

active

044865341

ABSTRACT:
A process for the preparation of immunologically active tagged conjugates by covalent linking of an enzyme with an antigen or haptene, which process comprises placing a solution of a mixture of immunologically active and inactive conjugates in contact with an insoluble complex former able to form a non-immunological complex reversibly with the untagged antigen or haptene component of the conjugate, separating the insoluble complex former, and eluting with a desorbent for untagged antigen or haptene.

REFERENCES:
patent: 3852157 (1974-12-01), Rubenstein et al.
patent: 4150033 (1979-04-01), Kitagawa
Kurger et al., An Introduction to Separation Science, John Wiley & Sons, N.Y. (1973), pp. 402-406.
Aurameas et al., "Coupling of Enzymes to Antibodies and Antigens", Scand. J. Immunol. vol. 8, Suppl. 7 (1978), pp. 7-23.

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