Dry analytical element having a spread control area for assaying

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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435291, 435805, 435810, 422 56, 422 57, 422 58, 422 60, 422 61, 422 68, 436808, 436810, C12Q 100, C12Q 148, G01N 3350, G01N 3090

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active

048897973

ABSTRACT:
A dry analytical element for assaying an enzyme in a liquid is disclosed, comprising a support having provided thereon at least a porous liquid-spreading layer composed of water non-absorbent fibers, wherein said porous liquid-spreading layer contains a substrate for an enzyme to be assayed and a hydrophilic polymer in an amount effective to decrease a spreading area by at least 20%. The analytical element exhibits high sensitivity and high reproducibility.

REFERENCES:
patent: 3867258 (1975-02-01), Forgione
patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4258001 (1981-03-01), Pierce et al.
patent: 4275031 (1981-06-01), Fischer et al.
patent: 4486537 (1984-12-01), Koyama et al.
patent: 4615983 (1986-10-01), Koyama et al.
D'Souza et al, "Multilaboratory Evaluation of Six Enzyme Tests on the Kodak Ektachem 700 Analyzer", (poster session XII International Congress of Clinical Chemistry), 1984.

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