Reagent comprising treated red blood cells and methods for detec

Details Reagent comprising treated red blood cells and methods for detec Reagent comprising treated red blood cells and methods for detec

1982-07-01

1986-05-06

Marantz, Sidney

Chemistry: analytical and immunological testing

For preexisting immune complex or auto-immune disease

Rheumatoid factors

424 3, 424 11, 436520, 436521, 436805, 436809, G01N 33555, G01N 33556, G01N 33564

Patent

active

045872228

ABSTRACT:
A reagent for the detection of rheumatoid factor in tests in tubes or microplates. The reagent contains a complex formed from red blood cells and from antibodies soluble with respect to these red blood cells, and immunologically fixed to the latter, said reagent being both stable and capable in given dilution intervals to give rise to hemagglutination only subject to contacting it with detectable concentrations of rheumatoid factor. The invention also relates to a process for making said reagent which comprises subjecting red cells and the antibodies to be fixed thereon to successive treatments with distinct aldehydes or tanning agents, as well as with proteinic substances, such as albumin, having dispersive properties. Finally the invention also provides a process for detecting the presence of rheumatoid factor in biological specimen which comprises contacting the latter with said reagent, whereby the response is positive or negative depending upon whether pattern hemagglutination or sedimentation of the cells of the complex is observed.

REFERENCES:
patent: 3322634 (1967-05-01), Fulthorpe
patent: 3548051 (1970-12-01), Dingwall
patent: 3594466 (1971-07-01), Guffroy
patent: 3708572 (1973-01-01), Peetoom
patent: 3828103 (1974-08-01), Fujita
patent: 3862302 (1975-01-01), Price
Milgrom, Arthritis & Rhev. vol. 7, 1964 pp. 1-7.
Williams, Method in Immunology and Immunochemistry, Acad. Press, New York, vol. 4, 1977, pp. 34-39.

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