Polypeptide expression using a portable temperature sensitive co

Details Polypeptide expression using a portable temperature sensitive co Polypeptide expression using a portable temperature sensitive co

1984-08-31

1987-05-19

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351723, 435243, 435317, 435849, 935 6, 935 8, 935 36, 935 39, C12N 120, C12N 1500, C12N 100, C12R 119

Patent

active

046668489

ABSTRACT:
The invention is a plasmid vector in which a first DNA sequence expressionable for a selected gene product is ligated to a positive retroregulatory element in a relationship thereto whereby expression of the selected gene product is enhanced. The first DNA sequence is furthermore operably linked to a second and third DNA sequence operably linked to one another which are respectively the P.sub.L promoter and a DNA sequence corresponding to an N gene ribosome binding site. Microorganisms transformed with the plasmid are also claimed.

REFERENCES:
patent: 4448885 (1984-05-01), Schneff et al.
patent: 4518584 (1985-05-01), Mark et al.
Maniatis et al. In Molecular Cloning, A Laboratory Manual, Cold Spring Harbor, 1982, pp. 405-411.
Boneigi et al, Cell 27:299-308, 1981.
Uhlin et al., Gene 6: 91-106, 1979.
Taniguchi et al., Nature 302:305-310, 1983.

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