Method of obtaining streptomyces protoplasts capable of efficien

Details Method of obtaining streptomyces protoplasts capable of efficien Method of obtaining streptomyces protoplasts capable of efficien

1977-07-01

1979-06-26

Tanenholtz, Alvin E.

Textiles: fiber preparation

195 78, 195 80R, 195 1, C12K 102

Patent

active

041592263

ABSTRACT:
A method of obtaining Streptomyces protoplasts which are able to regenerate viable cells with high efficiency is disclosed. The method involves growing Streptomyces cells to a particular physiological state, the transition phase between the exponential and stationary growth phases, and then forming protoplasts. The physiological state of the cells at the time of protoplast formation is crucial for efficient cell regeneration. Protoplasts obtained by this method enhance the use of protoplast-fusion techniques to effect genetic exchange within the genus Streptomyces, thereby facilitating the construction of hybrid or recombinant Streptomyces strains with useful properties.

REFERENCES:
okanishi et al., J. Gen. Microbiol, vol. 80, 389-400 (1974).
Villanueva et al., Yeast Mould and Plant Protoplasts, Academic Press, pp. 275-283 (1973).

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