Process for the activation of t-PA or Ing after genetic expressi

Details Process for the activation of t-PA or Ing after genetic expressi Process for the activation of t-PA or Ing after genetic expressi

1994-03-02

1995-09-26

Stone, Jacqueline M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 712, 435 695, 435 696, 435849, 435877, 530381, 5303871, 530402, 530404, 530412, 530414, 935 49, C07K 14745, C07K 1600, C12P 2102, C12N 1500

Patent

active

054533635

ABSTRACT:
A process for the activation of t-PA or IgG after expression in prokaryotes is described. The process includes cell digestion, solubilization under denaturing and reducing conditions and activation under oxidizing conditions in the presence of GSH/GSSG.

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