Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Patent
1992-07-20
1993-12-28
Johnston, Jill A.
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
552106, 552110, 552113, 552114, 552302, 436177, 2041801, 2041828, G01N 3300
Patent
active
052739066
ABSTRACT:
The present invention provides novel dyes for staining proteins, particularly those in electrophoretic gels. The dyes of the invention are derivatives of Coomassie.RTM. dyes. The invention also provides quantitative and qualitative methods for detecting proteins by staining the proteins with a dye of the invention.
REFERENCES:
patent: 2055607 (1936-09-01), Linch et al.
patent: 2085736 (1937-07-01), Calcott et al.
patent: 4023933 (1977-05-01), Bradford et al.
patent: 4219337 (1980-08-01), Grossberg et al.
patent: 4966854 (1990-10-01), Fleming
patent: 5013857 (1991-05-01), Berneth et al.
patent: 5101020 (1992-03-01), Fleming
In re Durden, 226 USPQ 359.
In re Pleuddermann, 15 USPQ 2nd 1738.
H. Schagger et al., "Coomassie Blue-Sodium dodeccyl Sulfate-Polyacrylamide Gel Electrophoresis, for Direct Visualization of Polypeptides during Electrophoresis", Anal. Biochem. 173, 201-205 (1988).
B. D. Hames, "Peptide mapping by Limited Proteolysis Using SDS-Polyacrylamide Gel Electrophoresis", in Gel Electrophoresis of Proteins--a Practical Approach.
B. Hames and D. Rickwood, eds., IRL Press, Washington, DC (1989), pp. 219-228.
B. Zehr et al., "A One-Step, Low Background Coomassie Staining Procedure for Polyacrylamide Gels", Anal. Biochem 182, 157-159 (1989).
Millipore Corp., "Direct Protein Microsequencing", Immobilon Tech Protocol (1987).
M. Hynkapiller et al., "Isolation of Microgram Quantities of Proteins from Poly-acrylamide Gels for Amino Acid Sequence Analysis", Meth. Enzymol. 91, 227-236 (1983).
C. Wilson "Staining of Proteins on Gels: Comparisons of Dyes and Procedures", Meth. Enzymol. 91, 236-247 (1983).
Leland David L.
Shultz John W.
Johnston Jill A.
Promega Corporation
Wallenhorst Maureen M.
LandOfFree
Protein staining compositions and methods does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Protein staining compositions and methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Protein staining compositions and methods will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1542177