Method and kit for performing nucleic acid hybridization assays

Details Method and kit for performing nucleic acid hybridization assays Method and kit for performing nucleic acid hybridization assays

1991-11-20

1993-12-28

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 536 243, C12Q 170, C12Q 168, C07H 2104

Patent

active

052738825

ABSTRACT:
A method and a kit for the isolation and quantitative detection of a selected target nucleic acid sequence from solution employing two probes. A first probe is complementary to one portion of the target and is covalently attached to a first complexing agent (e.g., either an antigen or an antibody). The second probe is complementary to a different portion of the target and is associated with a reporter group. Following hybridization of the target and two probes in solution, a solid support coated with a second complexing agent (i.e., a corresponding antibody or antigen) capable of binding to the first complexing agent on the first probe is employed to immobilize the target-probe hybrid complex. A plurality of types of first probes may be used. Each type is attached to the same sort of complexing agent but each includes a nucleic acid sequence which is complementary to a different portion of the target.

REFERENCES:
patent: 4256834 (1981-03-01), Zuk et al.
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4376110 (1983-03-01), David et al.
patent: 4455380 (1984-06-01), Adachi
patent: 4478914 (1984-10-01), Giese
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4495296 (1985-01-01), Neurath et al.
patent: 4556643 (1985-12-01), Paau et al.
patent: 4563417 (1986-01-01), Albarella et al.
patent: 4581333 (1986-04-01), Kourilsky et al.
patent: 4582789 (1986-04-01), Sheldon, III et al.
patent: 4894325 (1990-01-01), Englehardt et al.
Chooi, et al., Mol. Gen. Genet., 182, 245-251 (1981).
Dunn, et al., Cell, 12, 23-36 (1977).
Hu, et al., Gene, 17, 271-277 (1982).
Inouye, et al., J. Biol. Chem., 248(23), 8125-8129 (1973).
Ishikawa, et al., Scand. J. Immunol., 8, 43-55 (1978).
Johnson, et al., Gene Anal. Techn., 1, 3-8 (1984).
Leary, et al., Proc. Natl. Acad. Sci. (USA), 80, 4045-4049 (1983).
Maniatis, et al., Cell, 15, 687-701 (1978).
Manning, et al., Chromosoma (Berl.), 53, 107-117 (1975).
Manning, et al., Biochemistry, 16, 1364-1370 (1977).
Miller, et al., Biochim. Biophys. Acta, 366, 188-198 (1974).
Ranki, et al., Curr. Top. Microbiol. Immunol., 104, 307-318 (1983).
Ranki, et al., Gene, 21, 77-85 (1983).
Roizman, et al., Curr. Top. Microbiol. Immunol., 104, 273 (1983).
Salomon, et al., Biochemistry, 14, 4046-4050 (1975).
Thomas, Proc. Natl. Acad. Sci. (USA), 77, 5201-5205 (1980).
Watson, et al., Science, 218, 381-384 (1982).
Maniatis, et al., Molecular Cloning, Cold Spring Harbor Laboratory, New York, pp. 109-112 (1982).
Bayer et al., Methods of Biochemical Analysis, Glick ed., vol. 26, Wiley & Sons, pp. 1-45 (1980).
Sutton et al., J. Immunol. Methods, 82(2) 215-224 (1985) Biosis Number 81023643.

Affiliated with

Also associated with

Rating

Method and kit for performing nucleic acid hybridization assays does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method and kit for performing nucleic acid hybridization assays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and kit for performing nucleic acid hybridization assays will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1542104