Immobilized biocatalysts

Details Immobilized biocatalysts Immobilized biocatalysts

1982-11-01

1985-05-21

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435161, 435182, C12N 1110, C12N 1104, C12P 706

Patent

active

045186936

ABSTRACT:
Biocatalysts such as microbial cells are immobilized by forming spherical gel beads containing the microbial cells from a hydrogel such as agar or carrageenan, incubating the beads for a time sufficient to permit the microbial cells to produce CO.sub.2 and thereby decrease resistance of the beads to diffusion, diffusing into the beads a monomer, cross-linking agent and accelerator and contacting the beads with a polyermization initiator to cause polymerization of the monomer. The polymerized monomer prevents breakup characteristic of hydrogels containing growing microbial cells. This method is particularly suitable for the immobilization of microbial cells for use in fermentation to produce ethanol.

REFERENCES:
patent: 3849253 (1974-11-01), Harvey et al.
patent: 3959251 (1976-05-01), Porath et al.
patent: 4038140 (1977-07-01), Jaworek et al.
patent: 4081329 (1978-03-01), Jaworek et al.
patent: 4138292 (1979-02-01), Chibata et al.
patent: 4338401 (1982-07-01), Cremonesi
patent: 4393136 (1983-07-01), Cheetham

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