System for high-speed measurement and sorting of particles

Details System for high-speed measurement and sorting of particles System for high-speed measurement and sorting of particles

1991-03-18

1993-04-20

Sikes, William L.

Electrical pulse counters, pulse dividers, or shift registers: c

Applications

Counting animate or inanimate entities

377 12, G01N 2700

Patent

active

052048845

ABSTRACT:
A system for sorting particles is arranged such that the presence or absence of multiple parameters is determined for each particle in a flow of particles, and a logic condition is determined based on the multiple parameters. Thereafter, particles are outputted from the system when the logic condition is present. Accordingly, multiparameter high-speed measurements of a rare subpopulation of particles (e.g. biological cells) can be made amidst a larger population of particles with differing characteristics. When attached to a multiparameter flow cytometer/cell sorter and microcomputer, the system allows multiparameter analysis of cells at rates in excess of 100,000 cells/sec. This system can be an outboard module attached to a commercially available or home-built flow cytometer.

REFERENCES:
patent: 4086631 (1978-04-01), Vick
patent: 4115806 (1978-09-01), Morton
patent: 4653078 (1987-03-01), Aritomi et al.

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