Method for cloning and producing the NaeI restriction endonuclea

Details Method for cloning and producing the NaeI restriction endonuclea Method for cloning and producing the NaeI restriction endonuclea

1992-05-12

1994-03-08

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4353201, 43525233, 43525235, 435193, 536 232, C12N 922, C12N 1555

Patent

active

052926516

ABSTRACT:
The present invention is directed to a method for cloning and producing the NaeI restriction endonuclease by 1) introducing the restriction endonuclease gene from Nocardia aerocolonigenes into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the NaeI restriction endonuclease activity, and 3) purifying the NaeI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the NaeI restriction endonuclease activity.

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