Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-05-10
1999-01-12
Guzo, David
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435366, 435368, 435369, 435370, 435371, 435372, 4353722, 4353723, 4353201, 435373, C12N 510, C12N 1510, C12N 1586
Patent
active
058587403
ABSTRACT:
The invention provides a novel retroviral packaging system, in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retrovirus are produced in infected cells. The methods of the invention include the use of the novel retroviral constructs to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation at high efficiencies. The invention is useful for the rapid production of high viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.
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Dull Thomas J.
Farson Deborah A.
Finer Mitchell H.
Qin Lu
Roberts Margo R.
Cell Genesys Inc.
Guzo David
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