NF-AT-interacting protein NIP45 and methods of use therefor

Details NF-AT-interacting protein NIP45 and methods of use therefor NF-AT-interacting protein NIP45 and methods of use therefor

1996-11-25

1999-01-12

Campbell, Bruce R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 29, 4353201, 435325, 536 235, 536 245, 935 9, 935 22, 935 60, 935 66, C07H 2104, C12N 1500, C12P 2106, C12Q 102

Patent

active

058587110

ABSTRACT:
Isolated nucleic acid molecules encoding a novel protein, NIP45, that interacts with members of the Nuclear Factor of Activated T cell (NF-AT) family of proteins, are disclosed. The invention further provides antisense nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals carrying a NIP45 transgene. The invention further provides isolated NIP45 proteins and peptides, NIP45 fusion proteins and anti-NIP45 antibodies. Methods of using the NIP45 compositions of the invention are also disclosed, including methods for detecting NIP45 protein or mRNA in a biological sample, methods of modulating NIP45 activity in a cell, and methods for identifying agents that modulate an interaction between NIP45 and an NF-AT family protein.

REFERENCES:
Stratagene 1993 catalog, p. 157.
K Theiler (1989) The House Mouse pp. 148-149.
J Watson et al (1987) Molecular Biology of the Gene p. 313.
Hillier et al., EST Database Accession No. AA063239 (dated 23 Dec. 1997).
Hillier et al., EST Database Accession No. AA063239 (dated 24 Sep. 1996).
Sasaki et al. EST Database Accession No. D49049 (dated 2 Aug. 1995).
Minobe et al. EST Database Accession No. D24170 (dated 7 Dec. 1994).
Minobe et al. EST Database Accession No. D24480 (dated 14 Jan. 1994).
Sasaki, EST Database Accession No. C20299 (dated 24 Oct. 1996).
Fujiwara et al., EST Database Accession No. C18242 (dated 2 Oct. 1996).
Xie, Genbank Database Accession No. HSU40215 (dated 5 Oct. 1996).
Casolaro, V., et al., "Inhibition of NF-AT-dependent transcription by NF -kappa B: implications for differential gene expression in T helper cell subsets", Proc Natl Acad Sci USA, vol. 92, pp. 11623-11627 (1995).
Ho, I-C. et al., "The Proto-Oncogene c-maf is Responsible for Tissue-Specific Expression of Interleukin-4", Cell, vol. 85, pp. 973-983 (1996).
Hodge, M.R. et al., "The proximal promoter of the IL-4 gene is composed of multiple essential regulatory sites that bind at least two distinct factors", Journal of Immunology vol. 154, No. 12, pp. 6397-6405 (1995).
Hodge, M.R. et al., "Hyperproliferation and dysregulation of IL-4 expression in NF-ATp-deficient mice", Immunity vol. 4, pp. 397-405 (1996).
Rooney, J.W. et al., "A common factor regulates both Th1-and Th2 -specific cytokine gene expression", EMBO Journal, vol. 13(3), pp. 625-633 (1994).
Rooney, J.W. et al., "Coordinate and cooperative roles for NF -AT and AP-1 in the regulation of the murine IL -4 gene", Immunity, vol. 2, pp. 473-483 (1995).
Tara, D. et al., "Characterization of the constitutive and inducible components of a T cell IL -4 activation responsive element", J. Immunol, 154(9), pp. 4592-4602 (1995).

Affiliated with

Also associated with

Rating

NF-AT-interacting protein NIP45 and methods of use therefor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with NF-AT-interacting protein NIP45 and methods of use therefor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and NF-AT-interacting protein NIP45 and methods of use therefor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1514269