Polynucleotides encoding human DNA ligase III and methods of usi

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4352523, 43525411, 4353201, 435325, 435183, 536 232, C12N 1552, C12N 1563

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058587055

ABSTRACT:
A human DNA Ligase III polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques are disclosed. Also disclosed are methods for utilizing such polypeptide via gene therapy for the treatment of disorders associated with a defect in DNA Ligase III. Antagonists against such polypeptides and their use as a therapeutic to destroy unwanted cells are also disclosed. Diagnostic assays to detect mutant DNA Ligase III genes are also disclosed.

REFERENCES:
Tomkinson et al. DNA ligase III is the major high molecular weight DNA joining activity in SV40-transformed human fibroblasts: normal levels of DNA ligase III activity in Bloom syndrome cells. Nucleic Acids Research. vol. 21, No. 23, pp. 5425-5430, Nov. 25, 1993.
Caldecott et al. An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III. Molecular and Cellular Biology. vol. 14, pp. 68-76, Jan. 1994.
Tomkinson et al. Three distinct DNA ligases in mammalian cells. The Journal of Biological Chemistry. vol. 266, No. 32, pp. 21728-21735, Nov. 15, 1991.
Auffray et al. Partial cDNA sequence in EST-STS database, Accession No. Z45685, Nov. 6, 1994.
Auffray et al. Partial cDNA sequence in EST-STS database, Accession No. T95824, Mar. 27, 1995.
Auffray et al. Partial cDNA sequence in EST-STS database, Accession No. D15404, Mar. 18, 1995.
Auffray et al. Partial cDNA sequence in EST-STS database, Accession No. F07530, Feb. 17, 1995.
Auffray et al. Partial cDNA sequence in STS-EST database, Accession No. F00233, Mar. 7, 1995.
Hillier et al. Partial cDNA sequence in STS-EST database, Accession No. T95919, Mar. 27, 1995.
Clark et al. Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins. Molecular Biology of the Cell. vol. 5, No. 12, pp. 1301-1310, Dec. 1994.

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