Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-09-26
1999-01-12
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, C12Q 168, C12Q 170
Patent
active
058586580
ABSTRACT:
A method of quantitating genomic DNA in a sample is provided. The method comprises the steps of adding to the sample a given amount of at least one nucleic acid as an internal standard, wherein the standard nucleic acid differs from the genomic DNA to be quantified in at least one detectable characteristic; amplifying the genomic DNA and the internal standard nucleic acid by means of a nucleic acid amplification process employing primers complementary to repetitive genomic sequences; determining as a first amount the amount of amplified genomic DNA, and determining as a second amount the amount of amplified standard nucleic acid; and determining from the first and second amounts, as a third amount, the amount of genomic DNA originally contained in the sample. Kits for performing the method and products substantially free of foreign DNA as determined by the method also are provided.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis et al.
patent: 5213961 (1993-05-01), Bunn
patent: 5457027 (1995-10-01), Nadeau
Derwent Publications Ltd., London, GB; AN 86-234649 XP002022980 "Detection of dna and/or rna involves hybridising single stranded dan and/or rna labelled probe comprising repetitive sequence in dna and/or rna and detecting label," & JP-A-61 162 752 (Sekisui Chem Ind KK) 23 Jul. 1986.
Diviacco, "A novel procedure for quantitative polymerase chain reaction by coamplification of of competitive templates", Gene, vol. 122, No. 2, (1992) pp. 313-320.
Batzer et al., "Structure and Variability of Recently Inserted Alu Family Members", Nucleic Acids Research, vol. 18, No. 23 (1990), pp. 6793-6798.
Stumph et al., "Genomic Structure and Possible Retroviral Origin of the Chicken CRI Repetitive DNA Sequence Family", Proc. Natl. Acad. Sci. USA, vol. 81, (1984), pp. 6667-6671.
Saiki et al., "Primer-Directed Enzymatic Amplification of DNA With a Thermostable DNA Polymerase", Reports, vol. 239, (1988), pp. 487-491.
Mullis et al., "Specific Synthesis of DNA in Virto Via a Polymerase-Catalyzed Chain Reaction", Methods in Enzymology, vol. 155, (1987), p. 351.
Jelinek et al., "Repetitive Sequences in Eukaryotic DNA and Their Expression", Ann. Rev. Biochem. (1982), pp. 814-843.
Barrett et al., "Large-Scale Production and Purification of Vaccinia Recombinant-Derived HIV-1 gp 160 and Analysis of Its Immunogenicity", Aids Research and Human Retroviruses, vol. 5, No. 2, (1989), pp. 159-171.
Stumph et al., "A Chicken Middle-Repetitive DNA Sequence Which Shares Homology With Mammalian Ubiquitous Repeats", Nucleic Acids Res., vol. 9, No. 20, (1981), pp. 5383-5397.
Per et al., "Quantification of Residual DNA in Biological Products", Clinical Chemistry, vol. 35, No. 9, (1989), pp. 1859-1860.
Robertson et al., "A Collaborative Study to Examine the Sensitivity and Reproducibility of Assays for the Detection of DNA in Biologicals Derived From Continuous Cell Lines", Biologicals, vol. 20, (1992), pp. 73-81.
Gilliland et al., "Analysis of Cytokine mRNA and DNA: Detection and Quantitation by Competitive Polymerase Chain Reaction", Proc. Natl. Acad. Sci., vol. 87, (1990), pp. 2725-2729.
Wang et al., "Quantitation of mRNA by the Polymerase Chain Reaction", Proc. Natl. Acad. Sci., vol. 86. (1989), pp. 9717-9721.
Porcher et al., "A Simplified Method for Determination of Specific DNA or RNA Copy Number Using Quantitative PCR and an Automatic DNA Sequencer", Resch. Report, vol. 13, No. 1, (1992), pp. 106-113.
Dorner Friedrich
Falkner Falko-Guenter
Haemmerle Thomas
Himmelspach Michele
Kohl Johann
Campbell Eggerton A.
Immuno Aktiengesellschaft
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