Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1994-06-14
1996-10-01
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, 435101, 435195, 435 203, 435223, 435224, 4352561, 435911, 435942, 536 174, 536 20, 536 551, 536 552, 436164, 436172, 514 27, 514 53, C12Q 134, C12N 930, C08B 3708
Patent
active
055610510
ABSTRACT:
The present invention is directed to a method for screening samples for the identification of agents exhibiting potential fungicidal and insecticidal activity for a wide variety of agricultural, medical and pharmaceutical uses. The method utilizes cells that comprise a plasmid-born CTS gene of Saccharomyces cerevisiae, which allows for over expression of chitinase. Compounds that inhibit a hydrolyic action on methyl-umbelliferyltriacetyl chitotriose, but which are not toxic to the cells are detected by decrease in conversion of substrate
REFERENCES:
Somers, P. J. B., et al., Method for the Detection and Quantitation of Chitinase Inhibitors in Fermentation Broths; Isolation and Insect Life Cycle Effect of A82516, The Journal of Antibiotics, vo. XL No. 12, pp. 1751-1756, (1987).
Ludwig et al, FEMS Microbiology Letters, vol. 69, pp. 61-66, 1990.
Milewski et al, Journal of General Microbiology, vol. 138, pp. 2545-2550, 1992.
Barrett-Bee et al, Journal of General Microbiology, vol. 130, pp. 1857-1861, 1984.
Cabib, Methods in Enzymology, vol. 161, (Bioruoss, Pt. B). pp. 424-426, 1988.
Nishimoto et al, Biological Abstracts, vol. 92, No. 10, Ref. #110946, 1992 (J. Antibiot, 44(7):716-722, 1991).
Leah et al, The Journal of Biological Chemistry, vol. 266, No. 3, pp. 1564-1573, Jan. 25, 1991.
Cabib et al, Journal of General Microbiology, vol. 138, pp. 97-102, 1992.
Tronsmo, Arne, et al., Detection and Quantification of N-Acetyl-.beta.-D-glcosaminidase, Chitobiosidase, and Endochitinase in Solutions and on Gels, Analytical Biochemistry 208, 74-79 (1993).
American Cyanamid Company
Harrington James J.
Mohamed Abdel A.
Webster Darryl L.
Weimar Elizabeth C.
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