Process for making oligonucleotides having modified internucleos

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

536 231, C07H 2100

Patent

active

056484801

ABSTRACT:
Novel oligonucleotides, method for improving the hybridization properties of oligonucleotides and novel processes for preparing 3'-phosphorylated oligonucleotides are disclosed.

REFERENCES:
patent: 4959463 (1990-09-01), Froehler et al.
patent: 5185444 (1993-02-01), Summerton et al.
patent: 5235033 (1993-08-01), Summerton et al.
patent: 5264566 (1993-11-01), Froehler et al.
Mag et al.(I), "Synthesis and Selective Cleavage of an Oligodeoxynucleotide Containing a Bridged Non-Chiral Internucleotide 3'-Phosphoramidate Linkage," Tett. Lett., 33(48), 7319-7322 (1992).
Kledrowski et al., "Parabolic Growth of a Self-Replicating Hexadeoxynucleotide Bearing a 3',5'-Phosphoramidate Linkage," Angew. Chem. Intl. Ed., 30(4), 423-426 (1991).
Mag et al.(II), "Synthesis and Selective Cleavage of Oligodeoxyribonucleotides Containing Non-chiral Internucleotide Phosphoramidate Linkages," Nucleic Acids Res., 17(15), 5973-5988 (1989).
Gryaznov et al.(I), "Synthesis of Oligodeoxyribonucleotides Containing Phosphoramidate Internucleotide Bonds with the Use of the Atherton-Todd Reaction," Bioorg. Khim.15(7), 994-996 (1989); Chem. Abstr., 112(11), p. 807, Abstr. No. 99092x (1990).
Azhayev et al.(I), "Synthesis of Phosphoramidate Analogues of Short Oligonucleotides," Nucleic Acids Research Symposium Series, No. 9, IRL Press, Ltd., London, UK, 1981, pp. 251-254.
Krayevsky et al., "Synthesis of Oligonucleotides with 5'.fwdarw.3' Phosphoamidoester Bonds," Nucleic Acids Research Symposium Series, No. 9, IRL Press, Ltd., London, UK, 1981, pp. 203-205.
Shabarova et al., "Chemical Development in the Design of Oligonucleotide Probes for Binding to DNA and RNA," Biochemie, 70, 1323-1334 (1988).
Gromova et al., "Interaction of EcoRII Restriction and Modification Enzymes with Synthetic Fragments of DNA. IV. DNA Duplexes with Phosphoramide and Pyrophosphate Internucleotide Bonds--Substrates for the Study of Single-Strand Breaks," Molekulyarnaya Biol., 20(1) 29-40 (1986) (translation into English, Plenum Press, 1986).
Zaitseva et al., "Aminonucleosides and their Derivatives. X. 2'-Deoxydinucleoside Phosphates and 2'-Deoxydinucleotides with Phosphoramide Bonds," Bioorg. Khim., 10(3), 410-407 (1984) (in Russian with English Abstract following).
Lohrmann et al., "Template-directed Synthesis of High Molecular Weight Polynucleotide Analogues," Nature, 261, 342-344 (1976).
Gibbs et al., "The Synthesis of Phsophoramidates from Silylphosphites and Azides," Tett. Lett., 1977(8), 679-682.
Azhayev et al.(II), "Synthesis of Phosphoramidate Analogs of Ribonucleoside Phosphates," Coll. Czech. Chem. Comm., 44, 792-798 (1979).
Mungall et al., "Use of Phosphorus Oxychloride in Synthesizing Nucleotides and Oligonucleotides," Nucleic Acids Research, 1(4), 615-627 (1974).
Greene et al., "Formation of Internucleotide 3'-5' Phosphoramidate Links by Direct Coupling of Phosphoryl and Amino Groups," Nucleic Acids Research, 2(7), 1123-1127 (1975).
Azhayev et al.(III), "Aminonucleosides and Their Derivatives. IX. Synthesis of Short Oligoribonucleotides with Phosphoramide Internucleotide Links," Bioorg. Khim., 8(9), 1218-1224 (1982); Chem. Abstr., 98(5), p. 679, Abstr. No. 3489g (1983).
Sokolova et al.(I), "A New Method for Synthesis of Oligodeoxyribonucleotides with Phosphoramide Internucleotide Bond," Bioorg. Khim., 10(1), 75-78 (1984); Chem. Abstr., 100(25), p. 646, Abstr. No. 210339b (1984).
Isagulyants et al., "Template-Directed Condensation of Oligodeoxyribonucleotide Phosphorimidazolides--The General Way of Synthesis of Natural and Modified DNA-Duplexes," Bioorg. Khim., 11(2), 239-247 (1985); Chem. Abstr., 104(1), p. 567, Abstr. No. 6130e (1987).
Gryaznov et al.(II), "The Use of the Atherton-Todd Reaction for Synthesis of Oligodeoxyribonucleotides with Phosphorus Amide Internucleotide Bonds," Vestn. Mosk. Univ., Ser. 2: Khim., 27(4), 421-424 (1986); Chem. Abstr., 108(5), p. 680 Abstr. No. 38263z (1988).
Letsinger et al.(I), "Enzymatic Synthesis of Polydeoxyribonucleotides Possessing Internucleotide Phosphoramidate Bonds," J. Am. Chem. Soc., 94, 292-293 (1972).
Letsinger et al.(II), "Phosphoramidate Analogs of Oligonucleotides," J. Org. Chem., 35(11), 3800-3803 (1970).
Mungall et al., "Use of the Azido Group in the Synthesis of 5' Terminal Aminodeoxythymidine Oligonucleotides," J. Org. Chem., 40(11), 1659-1662 (1975).
Greene et al., "Formation of Internucleotide 3'-5' Phosphoramidate Links by Direct Coupling of Phosphoryl and Amino Groups," Nucleic Acids Research, 2(7), 1123-1127 (1975).
Zielinski et al.(I), "Oligomerization of Activated Derivatives of 3'-Amino-3'-deoxyguanosine on Poly(C) and Poly(dC) Templates," Nucleic Acids Research, 13(7), 2469-2484 (1985).
Zielinski et al.(II), "Oligoaminonucleoside Phosphoramidates. Oligomerization of Dimers of 3'-Amino-3'-deoxynucleotides (GC an CG) in Aqueous Solution," Nucleic Acids Research, 15(4), 1699-1715 (1987).
Zielinski et al.(III), "Autocatalytic Synthesis of a Tetranucleotide Analogue," Nature, 327, 346-347 (1987).
Bannwarth, "166. Solid-Phase Synthesis of Oligodeoxynucleotides Containing Phosphoramidate Internucleotide Linkages and Their Specific Chemical Cleavage," Helv. Chim. Acta 71, 1517-1527 (1988).
Sproat et al., "The Synthesis of Protected 5'-Amino-2',5'-dideoxyribonucleosides-3'-O-phosphoramidites; Applications of 5'-Amino-oligodeoxyribonucleotides," Nucleic Acid Research, 15(5), 6181-6196 (1987).
Letsinger et al.(III), "Incorporation of 5'-Amino-5'-deoxythymidine 5'-Phosphate in Polynucleotides by Use of DNA Polymerase I and a .phi.X174 DNA Template," Biochemistry, 15(13), 2810-2816 (1976).
Letsinger et al., "Enzymatic Synthesis of Duplex Circular .phi.X174 DNA Containing Phosphoramidate Bonds in the (-) Strand," Nucleic Acids Research, 3(4), 1053-1063 (1976).
Gryaznov et al.(III), "A New Method for the Synthesis of Oligodeoxyribonucleotides Containing Internucleotide Phosphoramidate Bonds," Tetrahedron Letters, 31(22), 3205-3208 (1990).
Barone et al., "In situ Activation of Bis-dialkylaminophosphines--A New Method for Synthesizing Deoxyoligonucleotides on Polymer Supports,"0 Nucleic Acids Research, 12(10), 4051-4061 (1984).
Agrawal et al., "Oligodeoxynucleoside Phosphoramidates and Phosphorothioates as Inhibitors of Human Immunodeficiency Virus," Proc. Nat. Acad. Sci. USA, 85, 7079-7083 (1988).
Froehler et al., "Nucleoside H-Phosphonates: Valuable Intermediates in the Synthesis of Deoxyoligonucleotides," Tetrahedron Letters, 27(4), 469-472 (1986).
Miller et al., "Synthesis of Oligo-2'-deoxyribonucleoside Methylphosphonates," Ch. 6 in Oligonucleotides and Analogues: A Practical Approach, Eckstein (ed.), Oxford University Press, London, UK, 1991, pp. 137-154.
Alul et la., "Oxalyl-CPG: A Labile Support for Synthesis of Sensitive Oligonucleotide Derivatives," Nucleic Acids Research, 19(7), 1527-1532 (1991).
Sokolova et al.(II), "Chemical Reactions with DNA Duplexes. Cyanogen Bromide as an Effective Oligodeoxyrobonucleotide Coupling Agent," FEBS Letters, 232(1), 153-155 (1988).
Dolinnaya et al., "Site-Directed Modification of DNA Duplexes by Chemical Ligation," Nucleic Acids Research, 16(9), 3721-3738 (1988).
Zon, "Oligonucleotide Analogues as Potential Chemotherapeutic Agents," Pharmaceutical Research, 5(9), 539-549 (1988).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Process for making oligonucleotides having modified internucleos does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for making oligonucleotides having modified internucleos, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for making oligonucleotides having modified internucleos will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1493181

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.