Expression of functional antibody fragments

Details Expression of functional antibody fragments Expression of functional antibody fragments

1995-05-03

1997-07-15

Feisee, Lila

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 696, 435 7021, 435 711, 4351723, 435972, 5303871, 5303873, C12P 2106, C12P 2104, C12P 2108, C12N 1513

Patent

active

056482370

ABSTRACT:
Methods for the high yield production of antibody Fv-containing polypeptides, especially Fab' and F(ab').sub.2 antibody fragments are provided. Expression of heavy and light chain Fv in a microbial secretory system is followed by recovery of Fv from the periplasm under conditions that maintain a cysteine residue as a free thiol. The free thiol is reacted with free thiol of an antibody fragment of the same or differing specificity, or with agents such as diagnostic labels or therapeutic moieties. The products offer advantages of homogeneity and purity not available through the use of known methods for preparing such derivatives.

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