Microbiological best method and reagents

Details Microbiological best method and reagents Microbiological best method and reagents

1996-04-18

1997-07-15

Kight, John

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 435 4, 435 8, 435 15, 4352831, 4352881, 4352883, 422 681, C12Q 102, C12Q 166, C12Q 148

Patent

active

056482329

ABSTRACT:
A method for detecting the presence and/or mount of microorganisms is described by adding adenosine diphosphate (ADP) to a sample suspected of containing microorganisms and/or their intracellular material, determining the amount of adenosine triphosphate (ATP) generated by adenylate kinase present, for example using a bioluminescent assay involving luciferase and luciferin, and relating the results to the presence and/or amount of microorganism. Test kits and apparatus for use in the method are also described.

REFERENCES:
patent: 3933592 (1976-01-01), Clendenning
patent: 4303752 (1981-12-01), Kolehmainen et al.
patent: 5004684 (1991-04-01), Simpson et al.
Kahru et al, "Microbios", vol. 62, pp 83-92, 1990.
J. Of Biochemical and Biophysical Methods 1 (1979) 163-169 Brolin et al "Photokinetic Microassay of Adenylate Kinase Using the Firefly Luciferase Reaction".
Chemical Abstracts vol. 96, 1982 p. 350 177160k "Determination of adenosine nucleotides . . . ".

Affiliated with

Also associated with

Rating

Microbiological best method and reagents does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Microbiological best method and reagents, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Microbiological best method and reagents will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1491721