Methods for producing thrombopoietin polypeptides using a mammal

Details Methods for producing thrombopoietin polypeptides using a mammal Methods for producing thrombopoietin polypeptides using a mammal

1994-11-30

1997-06-24

Walsh, Stephen

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 695, 435 698, 4351723, 4352542, 43525421, 43525423, 435325, 435419, 435348, 435349, 435352, 435359, 536 234, 935 10, 424 851, C12N 1519, C12N 1562, C07H 2104

Patent

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056416555

ABSTRACT:
DNA constructs useful in the production of thrombopoietin are disclosed. In general, the DNA constructs comprise a first DNA segment encoding a fusion of an amino-terminal secretory peptide joined to a thrombopoietin polypeptide and one or more additional DNA segments that provide for the transcription of the first segment. The secretory peptide is a native mammalian t-PA secretory peptide or may be modified to enhance proteolytic cleavage of the fusion. Also disclosed are cultured eukaryotic cells containing these DNA constructs and methods for producing thrombopoietin polypeptides through the use of the DNA constructs and cultured eukaryotic cells.

REFERENCES:
patent: 5037743 (1991-08-01), Welch et al.
patent: 5171844 (1992-12-01), van Ooyen et al.
patent: 5284755 (1994-02-01), Gearing et al.
D.T. Berg et al. Biochem. Biophys. Res. Commun. 179(3):1289-1296, Sep. 30, 1991.
Sohma et al., FEBS Letters 353:57-61 1994.
Krasney et al., Cytokine 4(2):134-143, Mar. 1992.
J.M. Cregg et al., Bio/Technology 11:905-910, Aug. 1993.
de Sauvage et al., Nature 369: 533-538, 1994.
Bartley et al., Cell 77: 1117-1124, 1994.
Lok et al., Nature 369: 565-568, 1994.
Pennica et al., Nature 301: 214-221, 1983.
U.S. Patent Application Serial No. 08/196,025 filed Feb. 14, 1994.
U.S. Patent Application Serial No. 08/203,197 filed Feb. 25; 1994.
U.S. Patent Application Serial No. 08/215,203 filed Mar. 21, 1994.
U.S. Patent Application Serial No. 08/252,491 filed Jun. 1, 1994.
U.S. Patent Application Serial No. 07/998,972, fild Dec. 20, 1992.

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