Method for determining the antimicrobial agent sensitivity of a

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 34, 435 4, 435 30, 435 36, 435 42, 435 29, 436 63, 436 74, 436 3, C12Q 102, C12Q 104, C12Q 118, G01N 3348

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active

056416458

ABSTRACT:
A method for determining the sensitivity of at least one nonparaffinophilic microorganism from a specimen obtained from a patient to different antimicrobial agent, and predetermined quantities thereof. The method includes providing at least one receptacle containing an aqueous solution and adjusting the solution to mimic the in vivo clinical conditions of said patient. The method further includes inoculating the solution with the specimen and placing into the receptacle (i) a slide coated with a carbon source and (ii) a predetermined quantity of an antimicrobial agent to be tested. By observing the nonparaffinophilic microorganism growth or lack thereof on the slide, it can be determined whether the predetermined quantity of the antimicrobial agent is effective in inhibiting growth of the nonparaffinophilic microorganism on the slide. An associated apparatus is also disclosed.

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