Removal of heparin from blood plasma samples using an insoluble

Details Removal of heparin from blood plasma samples using an insoluble Removal of heparin from blood plasma samples using an insoluble

1978-08-04

1980-04-22

Danison, Walter C.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

210 28, 210DIG23, 260112R, 424 2, 424101, 424177, C07G 700, A61K 3708

Patent

active

041995023

ABSTRACT:
This invention relates to a method for the removal of heparin from heparin-containing blood plasma test samples using an insoluble protamine reaction product, without adversely affecting subsequent testing of the plasma for clotting time. Protamine sulfate or a combination of protamine sulfate and serum albumin is cross-linked with glutaraldehyde to form an insoluble reaction product which is capable of adsorbing heparin. According to the method of this invention, excess amounts of either of the cross-linked protamine reaction products are added to blood plasma samples containing heparin and the mixture is agitated for a time sufficient to permit adsorption of substantially all heparin present. The insoluble protamine heparin complex formed is removed from the plasma along with any excess insoluble protamine reaction product. Aliquots of the heparin-free plasma may be subjected to coagulation tests in order to determine true clotting time.

REFERENCES:
patent: 4009267 (1977-02-01), Huber et al.
Franz et al., Chemical Abstracts, vol. 82 :15,032d, (1975).
Hall et al., Chemical Abstracts, vol. 84 :118,156t, (1976).
Allen et al., J. Lab. Clin. Med., vol. 34, (1949), pp. 473-476.
Habeeb et al., Arch. Biochem. Biophys., vol. 126, (1968), pp. 16-26.

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