Method of constructing a replicable cloning vehicle having quasi

Details Method of constructing a replicable cloning vehicle having quasi Method of constructing a replicable cloning vehicle having quasi

1979-07-05

1982-08-03

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 435 70, 435317, 536 27, C12N 1500

Patent

active

043428320

ABSTRACT:
Described are methods and means for the construction and microbial expression of quasi-synthetic genes arising from the combination of organic synthesis and enzymatic reverse transcription from messenger RNA sequences incomplete from the standpoint of the desired protein product. Preferred products of expression lack bio-inactivating leader sequences common in eukaryotic expression products but problematic with regard to microbial cleavage to yield bioactive material. Illustrative is a preferred embodiment in which a gene coding for human growth hormone (useful in, e.g., treatment of hypopituitary dwarfism) is constructed and expressed.

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