Expression and purification of cloned alpha-fetoprotein

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 691, 530350, 530380, 536 235, C12P 2106, A61K 3514, C12N 1500

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053842502

ABSTRACT:
In general, the invention features a method for producing human alpha-fetoprotein in prokaryctic cells. The method includes: providing a transformed prokaryotic cell including a recombinant DNA molecule encoding human alpha-fetoprotein operably linked to sequence elements capable of directing expression of alpha-fetoprotein, and permitting the transformed cell to express human alpha-fetoprotein.

REFERENCES:
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Innis et al., Arch. Biochem. Biophys 195:128, 1979.
Moringa et al., Proc. Natl. Acad. Sci. USA 80:4604, 1983.
Boismenu et al., Life Sciences 43:673, 1988.
Nishi et al., J. Biochem. 104:968 (1988).
Giuliani et al., Protein Engineering 2:605 (1989).
Yamamoto et al., Life Sciences 46:1679, (1990).

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