Sialic acid determination method

Details Sialic acid determination method Sialic acid determination method

1981-07-06

1982-08-03

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

252408, G01N 3352

Patent

active

043425674

ABSTRACT:
A method for determining sialic acid in blood plasma consisting essentially of the steps of precisely diluting the sample and cooling it, extracting with a mixture of chloroform and methanol, adding water to the mixture and separating the phases, adding phosphotungstic acid to the upper layer, centrifuging the resulting mixture and removing the supernatant, suspending the resulting precipitate, adding resorcinol reagent and heating, cooling the reaction mixture and adding butyl acetate-n-butanol to obtain a blue color which can be compared with standard curves to make the determination.

REFERENCES:
patent: 4146603 (1979-03-01), Davidson
Chemical Abstracts, 84:40392v (1976).

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