Primers for amplifying APC gene sequences

Details Primers for amplifying APC gene sequences Primers for amplifying APC gene sequences

1996-06-03

1998-06-02

Marschel, Ardin H.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435 691, 435 912, 536 241, 536 231, 536 2431, 536 2432, 536 2433, 935 77, 935 78, C07H 2100, C07H 2102, C07H 2104, C12P 1934

Patent

active

057602077

ABSTRACT:
A two-pronged method for diagnosis of genetic diseases can detect mutations in about 87% of familial adenomatous polyposis (FAP) patients. One part of the diagnostic method employs in vitro protein synthesis from surrogate genes created by amplifying either cDNA or genomic DNA. The second part of the diagnostic method employs an allele-specific expression assay which distinguishes the amount of mRNA expressed in vivo from each of a patient's two alleles. These approaches are readily applicable to the diagnosis of other genetic diseases.

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