Method for secretory production of protein

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 694, 435 697, 435189, 530350, 536 231, 536 232, 536 237, C12P 2106, C12P 2104

Type

Patent

Status

active

Patent number

057598100

Description

ABSTRACT:
In producing a recombinant protein in the periplasm of Escherichia coli, the productivity of the target ecombinant protein can be promoted by coexpressing glutathione reductase artificially with the target recombinant protein.

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"Molecular cloning of cDNA encoding, 20 kDa variant human growth hormone and the alternative splicing mechanism", Biochimeda et Biophysica Actd 949 (1988) 125-131, Dept. of Biochemistry, Mie University School of Medicine, Tsu, Mie (Japan), Naoki Masuda et al.
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Uchida et al. "Secretion of authentic 20-kDa human growth hormone (20 K HGH in Escherichia coli and properties of the purified product" J. Biotechnol. 55, 101-112, Jun. 1997.

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