Hybrid biosensors

Details Hybrid biosensors Hybrid biosensors

1997-05-30

1999-11-09

Redding, David A.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

4351731, 4351734, 4352887, 324447, 324692, C12M 300

Patent

active

059812680

ABSTRACT:
The invention relates to an apparatus and method for monitoring cells and to a method for monitoring changes in cells upon addition of an analyte to the cell's environment, comprising a device which includes an array of microelectrodes disposed in a cell culture chamber, upon which array a portion of cells adhere to the surfaces of the microelectrodes. The diameter of the cells are larger than the diameters of the microelectrodes. A voltage signal is applied across each of the microelectrodes and a reference electrode. Detection and monitoring of the signals resulting from the application of the voltage signal provides information regarding the electrical characteristics of the individual cells, including impedance (combined cell membrane capacitance and conductance), action potential parameters, cell membrane capacitance, cell membrane conductance, and cell/substrate seal resistance. Such an invention is useful in detecting or screening a variety of biological and chemical agents.

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