Electrophoresis of nucleic acid fragments

Details Electrophoresis of nucleic acid fragments Electrophoresis of nucleic acid fragments

1996-04-16

1998-12-15

Myers, Carla J.

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204469, 204456, 204450, 25218211, 25218212, 435 6, G01N 2726, C12Q 168

Patent

active

058491660

ABSTRACT:
A method for electrophoresis of nucleic acid fragments present in the solution which contains an amount, e.g., 0.2% or more, of a reagent, e.g., glycerol, dithiolthreitol (DTT) and trehalose or other sugars, which interact to form a complex with borate or boric acid. The method includes applying the solution to an electrophoretic gel and electrophoresing those fragments into the gel in the presence of a buffer lacking boric acid, or a derivative thereof, which forms a chelate complex with the reagent and thereby causes distortion of electrophoresis of the fragments in a gel including such a buffer.

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