Method for detecting Porphyromonas gingivalis

Details Method for detecting Porphyromonas gingivalis Method for detecting Porphyromonas gingivalis

1995-01-11

1999-11-09

Sisson, Bradley L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435212, 435810, 435822, C12Q 100, C12N 948

Patent

active

059811641

ABSTRACT:
A rapid and simple method and kit for the analysis of Porphyromonas gingivalis in a sample. A sample suspected of containing P. gingivalis is assayed for enhanced proteolytic, amidolytic, or esterolytic activity in the presence of a specific enhancer compound. Specifically, the enhancer is a mono- or di-peptide containing glycine, or an amide or ester thereof.

REFERENCES:
Z. Chen et al., "Stimulation of Proteinase and Amidase Activities in Porphyromonas (Bacteroides) gingivalis by Amino Acids and Dipepties," Infection and Immunity, vol. 59, No. 8, pp. 2846-2850, Jul. 1991.
Frankfater A. et al.; Biochemistry 1981, vol. 20, pp. 5517-5524.
Loeche W.J. et al.; J. Periodontolgy; 1990; vol. 61; No. 3; pp. 189-196.
Bajkowski A.S. et al.; The Journal of Biologic Chemistry; 1983, vol. 258; No. 3; pp. 1645-1549, 1650-1655.
Chemical Abstracts, vol. 108, No. 7, Abstract No. 53708s.
Bretz, et al., J. Dent. Res., vol. 66, No. 11, 1987, pp. 1668-1672.
Ono, et al., Oral Microbiol. Immunol., 1987: 2:77-81.

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