Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1983-07-18
1986-07-08
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
2504612, 356 39, 436 63, 436172, 436519, G01N 3350, G01N 33533
Patent
active
045993076
ABSTRACT:
A method for identifying subpopulations of cells of interest without interference from other cells. In the method, a sample of at least three (3) types of cells, including a first and second type of cell which form a subpopulation of cells of interest and a third type of cell which interferes with the identification of said subpopulation, is divided into at least two (2) aliquots. A first antibody which is specific for the third type of interfering cells but not for the subpopulation, is labeled with two (2) fluorochromes, each of the fluorochromes having distinct emission spectra. The labeled first antibody is then added to a first one of the sample aliquots so as to label the third type of cells with the first antibody. A first analysis is performed by analyzing each cell to determine the fluorescence emitted and to count the third type of cells so as to distinguish the third type of cells from the subpopulation. The information obtained from the first analysis is retained for subsequent use. The above steps are repeated for the subpopulation of cells of interest and a second analysis is obtained. The retained information from the first analysis is used to remove the effect of the third type of interfering cells and to provide a direct proportional analysis of the first and second types of cells in the subpopulation of cells of interest.
REFERENCES:
patent: 3826364 (1974-07-01), Bonner
patent: 3883247 (1975-05-01), Adams
patent: 3916205 (1975-10-01), Kleinerman
patent: 4243318 (1981-01-01), Stohr
patent: 4284412 (1981-08-01), Hansen
patent: 4336029 (1982-06-01), Natale
"Techniques in Immunocytochemistry", vol. 1, G. R. Bullock et al., eds., Chapt. Entitled `Applications of Double-Label Immunofluorescence` by K. B. Pryzwansky, pp. 77-89, Academic Press, New York, 1982.
Chang Chin-Hai
Saunders Alex M.
Becton Dickinson and Company
Marantz Sidney
McBride James R.
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