Optics: measuring and testing – Sample – specimen – or standard holder or support – Fluid containers
Patent
1995-09-27
1998-03-10
Pham, Hoa Q.
Optics: measuring and testing
Sample, specimen, or standard holder or support
Fluid containers
356440, 422 8205, 422 8209, G01N 110
Patent
active
057267510
ABSTRACT:
The present invention provides a flow cytometer made of two components: a flow cytometer optical head and a disposable flow module. The flow module utilizes a V-groove flow channel micromachined in a silicon wafer. The optical head comprises a laser to provide an illuminating beam and small and large angle photodetectors. Anisotropically etched facets of the V-groove reflect the illuminating beam. Small angle scattered light is also reflected by the V-groove wall and is collected by the small angle photodetector. Large angle scattered light and fluorescent light can exit the channel without reflection and are collected by the large angle photodetector. In addition, fluorescent light can be back-reflected by the V-groove, which enhances the collection efficiency.
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Altendorf Eric H.
Yager Paul
Pham Hoa Q.
University of Washington
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