Non-radioactive labelled single-stranded probe, method for manuf

Details Non-radioactive labelled single-stranded probe, method for manuf Non-radioactive labelled single-stranded probe, method for manuf

1989-07-11

1990-12-18

Wax, Robert A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435 7, 435235, 435239, 435320, 436501, 530387, 536 27, 935 73, 935 78, 935 12, 935110, C07H 2104, C07H 502, C12Q 168, G01N 33531, C12N 1511

Patent

active

049787497

ABSTRACT:
The invention relates to a new probe for detecting a specified nucleic acid sequence. It consists of a recombinant DNA containing an insert complementary to the nucleic acid sequence to be detected and nuclecic acid sequences derived from the DNA (+) of phage M13, the bases which participate in the composition of this recombinant DNA, in particular the thymine groups, being replaced by 5-bromouracil groups. The invention also relates to a method for manufacturing this probe, this method comprising the culturing of cell hosts, more especially E. coli, which are auxotrophic for thymine in a medium containing a limiting concentration of thymine and containing 5-bromouracil. After the requisite incubation time, the supernatant is removed and the probe is recovered from the bacterial cells.

REFERENCES:
patent: 4529700 (1985-07-01), Gratzner
patent: 4585736 (1986-04-01), Dolbeare et al.
patent: 4687732 (1987-08-01), Ward et al.
Sakamoto et al. (1987) Molecular and Cellular Probes, vol. 1, pp. 109-120.
Traincard et al. (1983) Ann., Imnunol. (Inst. Pasteur), vol. 134D, pp. 399-405.
Gratzer, H. G. (1982) Science 218, pp. 474-475.
Stratis, A., et al., European Patent Application No. 0,143,059-Al, Filed 11/12/84.

Affiliated with

Also associated with

Rating

Non-radioactive labelled single-stranded probe, method for manuf does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Non-radioactive labelled single-stranded probe, method for manuf, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Non-radioactive labelled single-stranded probe, method for manuf will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1426334