Methods and compositions for expression of BTI endotoxin

Details Methods and compositions for expression of BTI endotoxin Methods and compositions for expression of BTI endotoxin

1987-01-13

1990-12-18

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 435 711, 435 91, 435170, 4351721, 4351723, 4352523, 43525233, 435832, 435848, 536 27, 935 6, 935 9, 935 22, 935 23, 935 29, 935 59, 935 60, 935 64, 935 72, 935 73, 935 74, C12N 121, C12N 1532, C12N 1563, C12N 1570

Patent

active

049786237

ABSTRACT:
DNA sequences are provided coding for Bacillus thuringiensis var. israelensis (BTI) endotoxin, employing bacterial hosts which produce a protein having insecticidal activity for dipteran insects.
The bacteriophage lambda strain SYN A4-1 was deposited at the A.T.C.C. on Feb. 22, 1984, and given Accession No. 40098.

REFERENCES:
patent: 4467036 (1984-08-01), Schnepf et al.
patent: 4652628 (1987-03-01), Walfield et al.
Ward et al. 1984, "Cloning and Expression in E. coli of the Insecticidal .delta.endotoxin gene . . . ", FEBS v175(2) pp. 377-382.
Fitz-James 1982 "Symposium on B. thuringiensis var israelensis H-14: A bacterial insecticide", 7th Ann. Ontario Mosquito Control Association Meeting, Mar. 1-2, 1982.
Schnepf et al., 1981, "Cloning and Expression of the Bacillus thuringiensis crystal protein gene in E. coli", Proc. Natl. Acad. Sci. vol. 78, pp. 2893-2897.
Sekar et al., 1985, "Molecular Cloning of the delta-endotoxin gene of Bacillus thuringiensis var israelensis", Gene , vol. 33 pp. 151-158.
Gonzalez Jr., 1982, "Transfer of B. thuringiensis plasmids coding for .delta.-endotoxin . . . ", Proc. Natl. Acad. Sci., vol. 79 pp. 6951-6955.
Ward et al., 1983, "Assignment of the .delta.-endotoxin gene of Bacillus thuringiensis var israelensis to a specific plasmid . . . ", FEBS vol. 158 pp. 45-49.
Thorne et al. 1986, J. Bacteriol. 166(3):801-811.
Bourgouin et al. 1986 MGG 205:390-397.
Garduro et al., 1988, App. Environ. Microbiol. 54(1):277-279.
Ward et al., "Bacillus thuringiensis var. israelensis .delta.-Endotoxin", in J. Mol. Biol. (1986) 191:1-11.
Waalwijk, et al., "Molecular cloning and the nucleotide sequence of the M.sub.r crystal protein gene of Bacillus thuringiensis subsp. israelensis", in Nucleic Acids Research (1985) 13:8207-8217.

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